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Genetic knockout (KO) of Rac1 in excitatory neurons in mice resulted in decreased spine and synapse numbers accompanied by increased postsynaptic density (PSD) ( in vitro through constitutively active Rac1 in cultured hippocampal slices leads to increased spine density but reduced spine size (Nakayama et al., 2000

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Cocoa polyphenols induce the expression of SIRT1 and SIRT3 [145]

239 Moreover, pharmacokinetic limitations, including low bioavailability and rapid metabolism, must be carefully addressed when translating in vitro results to in vivo or clinical settings

is 100%, but is frequently lower after oral ingestion because the drug may be incompletely absorbed

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